Coding

Part:BBa_K3831001:Design

Designed by: Klára Odehnalová   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


BMC - pduB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 513
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in Bacillus subtilis and for compatibility with the Assembly Standards.


Source

Wade Y., Daniel R. A., Leak D. J. 2019. Heterologous Microcompartment Assembly in Bacillaceae: Establishing the Components Necessary for Scaffold Formation. ACS Synth. Biol. 8: 1642-1654. DOI: 10.1021/acssynbio.9b00155

References